Investigations of the Effects of Gender, Diurnal Variation and Age in Human Urinary Metabolomic Profiles
Metabolomics may have the capacity to revolutionize disease diagnosis through the identification of scores of metabolites that vary during environmental, pathogenic, or toxicological insult. NMR spectroscopy has become one of the main tools for measuring these changes since an NMR spectrum can accurately identify metabolites and their concentrations. The predominant approach in analyzing NMR data has been through the technique of spectral binning. However, identification of spectral areas in an NMR spectrum is insufficient for diagnostic evaluation, since it is unknown whether areas of interest are strictly caused by metabolic changes, or are simply artifacts. In this paper, we explore differences in gender, diurnal variation and age in a human population. We use the example of gender differences to compare traditional spectral binning techniques (NMR spectral areas) to novel targeted profiling techniques (metabolites and their concentrations). We show that targeted profiling produces robust models, generates accurate metabolite concentration data, and provides data that can be used to help understand metabolic differences in a healthy population. Metabolites relating to mitochondrial energy metabolism were found to differentiate gender and age. Dietary components and some metabolites related to circadian rhythms were found to differentiate time of day urine collection. The mechanisms by which these differences arise will be key to the discovery of new diagnostic tests and new understandings of the mechanism of disease.
Citation
C. Slupsky,
K. Rankin,
H. Fu,
D. Chang,
E. Saude,
B. Lix,
D. Adamko,
S. Shah,
R. Greiner,
B. Sykes,
T. Marrie.
"Investigations of the Effects of Gender, Diurnal Variation and Age in Human Urinary Metabolomic Profiles".
Analytical Chemistry, August 2007.
| Keywords: |
machine learning, Metabolomics, NMR, Targeted Profiling, Urine, Normal, Bioinformatics, Medical informatics |
| Category: |
In Journal |
| Web Links: |
Journal page |
BibTeX
@article{Slupsky+al:07,
author = {Carolyn Slupsky and K Rankin and H Fu and D Chang and E Saude and B
Lix and D Adamko and S Shah and Russ Greiner and Brian D. Sykes and Tom
Marrie},
title = {Investigations of the Effects of Gender, Diurnal Variation and Age
in Human Urinary Metabolomic Profiles},
journal = {Analytical Chemistry},
year = 2007,
}Last Updated: April 28, 2012
Submitted by Russ Greiner 
