View Publication

The Human Cerebrospinal Fluid Metabolome

David S. Wishart, Departments of Computing Science and Biology, University of Alberta
M D Lewis
J Morrisey
M Flegel
Y Xiong
Kevin Jeroncic
Dean Cheng
Roman Eisner
Bijaya Gautam
Dan Tzur
Summit Sawhney, Department of Biological Sciences, University of Alberta
Fiona Bamforth, Department of Medicine, University of Alberta
Russ Greiner, Dept of Computing Science; PI of AICML
Liang Li, Department of Chemistry, University of Alberta

With continuing improvements in analytical technology and an increased interest in comprehensive metabolic profiling of biofluids and tissues, there is a growing need to develop comprehensive reference resources for certain clinically important biofluids, such as blood, urine and cerebrospinal fluid (CSF). As part of our effort to systematically characterize the human metabolome we have chosen to characterize CSF as the first biofluid to be intensively scrutinized. In doing so, we combined comprehensive NMR, gas chromatography-mass spectrometry (GC-MS) and liquid chromatography (LC) Fourier transform-mass spectrometry (FTMS) methods with computer-aided literature mining to identify and quantify essentially all of the metabolites that can be commonly detected (with today's technology) in the human CSF metabolome. Tables containing the compounds, concentrations, spectra, protocols and links to disease associations that we have found for the human CSF metabolome are freely available at http://www.csfmetabolome.ca.

Citation

D. Wishart, M. Lewis, J. Morrisey, M. Flegel, Y. Xiong, K. Jeroncic, D. Cheng, R. Eisner, B. Gautam, D. Tzur, S. Sawhney, F. Bamforth, R. Greiner, L. Li. "The Human Cerebrospinal Fluid Metabolome". Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, August 2008.

Keywords:	Cerebrospinal fluid, CSF, Metabolome, Metabolomics, Nuclear magnetic resonance, Mass spectrometry, Metabolomics, bioinformatics, medical informatics
Category:	In Journal
Web Links:	DOI

BibTeX

@article{Wishart+al:J.ChromatographyB08,
  author = {David S. Wishart and M D Lewis and J Morrisey and M Flegel and Y
    Xiong and Kevin Jeroncic and Dean Cheng and Roman Eisner and Bijaya Gautam
    and Dan Tzur and Summit Sawhney and Fiona Bamforth and Russ Greiner and
    Liang Li},
  title = {The Human Cerebrospinal Fluid Metabolome},
  journal = {Journal of Chromatography B-Analytical Technologies in the
    Biomedical and Life Sciences},
  year = 2008,
}

Last Updated: April 27, 2012
Submitted by Russ Greiner

Not Logged In

PapersDB

The Human Cerebrospinal Fluid Metabolome

Citation

BibTeX